CCL5 anticorps
Aperçu rapide pour CCL5 anticorps (ABIN115981)
Antigène
Voir toutes CCL5 AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
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Purification
- Affinity chromatography.
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Immunogène
- Highly pure (>98%) recombinant rRANTES.
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Indications d'application
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Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofrRANTES (100 ng/mL), a concentration of 3.0-5.0 μg/mL of this antibody is required. ELISA: To detect rRANTES by direct ELISA (using 100l/well antibody solution) aconcentration of at least 0.5g/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with compatible secondary reagents, allows the detection of0.2-0.4 ng/well of recombinant rRANTES. Western Blot: To detect rRANTES by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant rRANTES is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
Restrictions
- For Research Use only
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Reconstitution
- Restore in sterile water to a concentration of 0.1-1.0 mg/mL.
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Buffer
- PBS, pH 7.2 without preservatives.
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Agent conservateur
- Without preservative
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Conseil sur la manipulation
- Avoid repeated freezing and thawing. Centrifuge vial prior to opening!
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Stock
- 4 °C/-20 °C
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Stockage commentaire
- Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.
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- CCL5 (Chemokine (C-C Motif) Ligand 5 (CCL5))
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Autre désignation
- RANTES / CCL5
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Sujet
- Synonyms: C-C motif chemokine 5, D17S136E, E °CP, Eosinophil-chemotactic cytokine, SCYA5, SIS-delta, Small-inducible cytokine A5, T-cell-specific protein RANTES, cell-specific protein P228
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ID gène
- 10116
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UniProt
- P50231
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Pathways
- Cellular Response to Molecule of Bacterial Origin, Regulation of G-Protein Coupled Receptor Protein Signaling, Smooth Muscle Cell Migration
Antigène
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